Solution-Bradford reagent and an equivalent volume

1. When preparing the protein standards, we used a tube (T) without protein, but including Bradford reagent and an equivalent volume of 0.15 M NaCl instead of protein. Can we just use plain water to prepare tube T instead?

2. The objective of this exercise was to train you in determining protein concentration in different extracts from biological samples. The protein concentration in a particular sample is used to standardize the activity of a protein such as an enzyme, a transporter, or receptor. Define the term “specific activity” in regards to an enzymatically catalyzed reaction.

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