Solution-Define restriction fragment length polymorphism

1. Severe combined immunodeficiency disease (SCID) is fatal at an early age. SCID is caused by the lack of the enzyme adenosine deaminase, which is encoded by the gene ADA. Suppose you want to use a gene therapy method to treat the disease. You have DNA that contains the ADA gene and a virus that infects human cells. Design a protocol to get the ADA gene into the viral DNA.

2. Define restriction fragment length polymorphism (RFLP) analysis. The information generated from RFLP is useful in criminal investigations. Suppose some DNA samples are taken from a crime scene, suspect, and the victim. The samples are amplified by PCR, and then subjected to RFLP analysis. Below is a gel electrophoresis of the RFLP results. D1 is from defendant 1; D2 is from defendant 2; V is from the victim; and S1, S2, and S3 are from the crime scene. Speculate on how these results would have an impact on each defendant.
3. Define genomics and proteomics. Name two techniques that are used in each field of study. Protein is translated from mRNA, which is transcribed from the gene. However, the proteome is much larger than the genome for a given organism. Give two reasons why there are more proteins than type of genes.

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