What would happen to plates poured with agar that is too hot?

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BIO2071_Microbiology Laboratory Review Sheet Week 2 Exercise 8 Questions 1. Define a culture medium. 2. Discuss some of the physical and chemical factors involved in the composition, and in the preparation, of a culture medium. Nutrient ingredients: ___________________________________________________________ ___________ pH and buffering: ___________________________________________________________ ___________ Heat (to reconstitute): ___________________________________________________________ ___________ Heat (to sterilize): ___________________________________________________________ ____________ Other: ___________________________________________________________ ____________ 3. At what temperature does agar solidify? ________________________________________________________________ _____________ Page 1 of 5 From Laboratory Manual & Workbook in Microbiology Applications to Patient Care (9th ed.). By Josephine A. Morello, Helen Eckel Mizer, and Paul A. Granato Copyright Š 2006 The McGraw-Hill Companies, Inc. Reprinted with permission of The McGraw-Hill Companies, Inc.

BIO2071_Microbiology Laboratory At what temperature does agar melt? ________________________________________________________________ _____________ 4. What would happen to plates poured with agar that is too hot? ________________________________________________________________ _____________ Could they be used? ________________________________________________________________ _____________ 5. What would happen to plates poured with agar that is too cool? ________________________________________________________________ _____________ Could they be used? ________________________________________________________________ _____________ 6. Why are culture media sterilized before use? 7. Discuss the relative value of broth and agar media in isolating bacteria from mixed cultures. 8. Are nutrient broths and agars, as you have prepared them, suitable for supporting growth of all microorganisms pathogenic for humans?…