Which is an inhibitor of mitochondrial atp synthesis

You have discovered a novel compound (U-500), which is an inhibitor of mitochondrial ATP synthesis. You observe that when U-500 is aIDedto cells or isolated mitochondria, the NAD+/NADH ratio decreases. Do you think that U- 500 is an uncoupling agent or an inhibitor of respiratory electrontransfer? Explain briefly, and suggest an aIDitional experiment that you could do toconfirm your hypothesis [Tip: assume you have access to an oxygen electrode,which you can use to measure rates of oxygen uptake by mitochondria].

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