Blood Glucose Experiment

Week 7 Lab Instructions
Blood Glucose Regulation
Activity
Deliverable
Points
Part A
Blood Glucose Experiment
30
References:
1. Tortora and Derrickson Introduction to the Human Body, 14th ed., Wiley, 2012.
2. Allen & Harper, Laboratory Manual for Anatomy and Physiology, 5th ed., Wiley, 2014.
Supplies:
· Glucometer
· Test Strips
· Alcohol Wipes
· Sugary snacks or glucose tablets
Part A
Step 1: Read the Entire Lab Packet
1.0– Read through the laboratory packet – SEE ATTACHED SHEETS and read pages 391 – 401 of Laboratory Manual for Anatomy & Physiology (5th Edition)
Part A
Step 2: Come to the Lab with Proper PPE
IMPORTANT NOTATION: 2 MEMBERS OF YOUR TEAM SHOULD COME TO LABORATORY IN A ‘FASTING STATE’ – no food or drinks for at least 3 hours prior to laboratory – only have members of your group without any health constraints participate in this component of the lab!!
2.0 – Follow the instructions provided by your professor
2.1 – Obtain needed equipment and supplies to be set up at your laboratory station
· gloves
· laboratory coat
· glucometer
· test strips
· lancets
· alcohol wipes
· high sugar content snacks or glucose tablets
2.3 – Follow the directions of the packet and as presented by your instructor conduct the experiment on glucose regulation
2.4 – Collect the data on two members of your laboratory team
2.5 – Share the results of your laboratory team with all other research teams in your class
2.6 – Analyze the results and write a formal lab report as outlined below
2.7 – Make sure to include the following in your report
a. – a tabular presentation of your data
b. – answers to the following questions related to sugar regulation in the blood.
i. Did the time that a person fasted have anything to do with the amount of increase of sugar in the blood following ingestion of the glucose tablets
ii. How quickly did the glucose levels rise in the blood following ingestion?
iii. Why was there a delay?
iv. How long did blood sugar remain elevated and why?
v. Did the weight of the subject have anything to do with the amount of increase in blood glucose? Why or Why not?
**LAB REPORT IS DUE PRIOR TO THE BEGINNING OF THE WEEK 8 FINAL EXAM**
Activity
Deliverable
Points
Part A
Blood Glucose Experiment
30
Note that the lab report should have the following format….
Title
Introduction: The first section of your lab report will be the introduction. In this paragraph or paragraphs, you are to provide information to the reader so that he or she can understand the purpose of the experiment.
· Purpose: A concise statement about the lab’s objective.
· Background: A brief summary of the topic you are investigating. Include any information that would be necessary to understand the stated purpose.
Procedure: This is the second section in your lab report. This section includes information that the reader would need to repeat your experimental procedure. Do not include any observations or results in this section.
Some questions that you should ask yourself to complete this section include: What was used? What equipment? How long did you perform a step within the procedure?
Observations and Results: This is the third section of your report. In this section, you will communicate what you observed during the experiment. The results section is typically dominated by calculations, tables, and figures. In your tables, label the axes of any graphs.
Discussion: This is the fourth section of your report. In this section, you will explain, analyze, and interpret your observations and results. Additionally, you will draw conclusions based on your existing knowledge. This also is where you demonstrate your understanding of the experiment by construing the significance or meaning of the results. This is the section where you should answer any accompanying questions with the laboratory procedure.
Conclusion: This is the last section of your report and is separate from the discussion section. In a few sentences, provide a concluding statement about the results of your lab. In a scientific publication, this section summarizes the significant aspects and results, and identifies implications for future study.
W7 Lab Worksheet
Blood Glucose Experiment
Watch the demonstration on how to correctly use the glucometer and the procedures for checking a blood sugar as conducted by your Professor.
One you feel confident in the procedure, conduct the experiment on two members of your group
CONTROL CONDITION:
–Measure glucose levels in both experimental subjects at the beginning of lab (while still fasting)
SUBJECT #1
WEIGHT
TIME SINCE LAST EATEN
GLUCOSE LEVEL
SUBJECT #2
WEIGHT
TIME SINCE LAST EATEN
GLUCOSE LEVEL
INGEST GLUCOSE TABLETS
WAIT 15 MINUTES
MEASURE GLUCOSE LEVELS
SUBJECT 1 – GLUCOSE LEVEL
SUBJECT 2 – GLUCOSE LEVEL
WAIT ADDITIONAL 15 MINUTES (TOTAL 30 MINUTES)
MEASURE GLUCOSE LEVELS
SUBJECT 1 – GLUCOSE LEVEL
SUBJECT 2 – GLUCOSE LEVEL
WAIT ADDITIONAL 30 MINUTES (TOTAL 45 MINUTES)
MEASURE GLUCOSE LEVELS
SUBJECT 1 – GLUCOSE LEVEL
SUBJECT 2 – GLUCOSE LEVEL
WAIT FINAL 15 MINUTES (TOTAL 1 HOUR)
MEASURE FINAL GLUCOSE LEVEL
SUBJECT 1 – GLUCOSE LEVEL
SUBJECT 2 – GLUCOSE LEVEL
Complete the following table for your entire class:
TIME (MINUTES)
SUBJECT#
TIME FASTING
Weight
CONTROL
15
30
45
60
Shana (glucose)1
6 1/2 hours
220
95
118
125
98
89
Rita (dark chocolate) 2
1 1/2 hours
98
89
100
98
110
88
Shannon (glucose) 3
½ hours
180
94
119
91
87
84
Chris (glucose& chocolate) 4
2 1/2 hours
130
88
107
92
88
85
Thornton (chocolate) 5
3 hours
155
89
91
106
90
87
6
7
8
AVERAGE
STD.DEV.